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Cover Image
Cover Image
On the cover
Compared with a control cell (top), cells lacking either the clathrin heavy chain CHC17 (right), clathrin light chains (bottom), or both (left) display an increased number of centrosomes (green) and a multipolar mitotic spindle (red). The clathrin light chains are stained blue. Foraker et al. reveal that clathrin promotes centrosome integrity by stabilizing the microtubule-binding protein ch-TOG.
Image © 2012 Foraker et al.
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In This Issue
In Focus
Clathrin helps maintain the centrosome's integrity
Inactivating clathrin in S phase reveals the protein's involvement in centrosome maturation.
People & Ideas
Bruce Nicklas: Pioneering studies on spindle forces
Nicklas performed innovative studies on the forces that drive cell division.
Review
Report
Polo-like kinase-1 regulates kinetochore–microtubule dynamics and spindle checkpoint silencing
Plk1 dynamics at kinetochores control two critical mitotic processes: initially establishing correct kinetochore–microtubule attachments and subsequently silencing the spindle checkpoint.
SCAR knockouts in Dictyostelium: WASP assumes SCAR’s position and upstream regulators in pseudopods
In the absence of SCAR, Dictyostelium WASP is relocalized from coated pits to the leading edge of the cell and activated to drive pseudopod formation.
Article
Open chromatin structures regulate the efficiencies of pre-RC formation and replication initiation in Epstein-Barr virus
Studies of EBV replication origins demonstrate an excess of pre-replication complexes that are formed at flexible MNase-sensitive sites in the genome.
Mammalian GW220/TNGW1 is essential for the formation of GW/P bodies containing miRISC
Localization of the miRNA-induced silencing complex to GW/P bodies by GW220/TNGW1 may regulate the fate of target mRNAs.
Rab10 and myosin-Va mediate insulin-stimulated GLUT4 storage vesicle translocation in adipocytes
Rab10 activation promotes GLUT4 storage vesicle recruitment to the plasma membrane in response to insulin and coordinates with myosin-Va to mediate vesicle fusion.
T granules in human platelets function in TLR9 organization and signaling
TLR9 localizes to a novel intracellular compartment called the T granule to promote immune signaling by platelets.
Ubiquitylation by Trim32 causes coupled loss of desmin, Z-bands, and thin filaments in muscle atrophy
During muscle atrophy induced by fasting, Trim32 promotes degradation of desmin cytoskeleton, which is linked to the loss of Z-bands and thin filaments.
Clathrin promotes centrosome integrity in early mitosis through stabilization of centrosomal ch-TOG
Clathrin inactivation during S phase destabilizes the microtubule-binding protein ch-TOG, affecting its centrosomal localization and centrosome integrity during early mitosis.
PKA and PDE4D3 anchoring to AKAP9 provides distinct regulation of cAMP signals at the centrosome
Control of cell cycle progression relies on unique regulation of centrosomal cAMP/PKA signals through PKA and PDE4D3 interaction with the A kinase anchoring protein AKAP9.
Hsp110 is required for spindle length control
The Hsp70–Hsp110 chaperone complex antagonizes Cin8 plus-end motility and prevents premature spindle elongation in S phase.
Fission yeast Ags1 confers the essential septum strength needed for safe gradual cell abscission
The α(1-3)glucan synthase Ags1 is essential for both secondary septum formation and the primary septum structural strength needed to counter cell turgor pressure during cell separation.
Mena binds α5 integrin directly and modulates α5β1 function
Mena binds to the cytoplasmic tail of α5 integrin and modulates key α5β1 integrin functions in adhesion, motility, and fibrillogenesis.
Epithelial junction formation requires confinement of Cdc42 activity by a novel SH3BP1 complex
Epithelial junction formation and morphogenesis requires the GTPase-activating protein SH3BP1 and the F-actin–capping protein CapZ to guide Cdc42 signaling and cytoskeletal dynamics.
Wnt-11 and Fz7 reduce cell adhesion in convergent extension by sequestration of PAPC and C-cadherin
Wnt-11/frizzled-7 reduces the lateral clustering of C-cadherin by capturing the protocadherin PAPC and C-cadherin into distinct adhesion-modulating complexes.
A steep phosphoinositide bis-phosphate gradient forms during fungal filamentous growth
A gradient of PI(4,5)P2 formed by phospholipid synthesis, diffusion, and regulated turnover is crucial for filamentous growth.
Hierarchical assembly of the eggshell and permeability barrier in C. elegans
Assembly of the trilaminar eggshell and development of the permeability barrier after fertilization in C. elegans are distinct in their timing and mechanism.
Tools
A novel split kinesin assay identifies motor proteins that interact with distinct vesicle populations
A novel split kinesin assay identifies three Kinesin-3 family members that specifically interact with dendritic vesicle populations in neurons.
