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Cover Image
Cover Image
On the cover
Bowen et al. reveal that septin filaments (green) guide the reorganization of microtubules (red) as a flat MDCK cell (bottom) polarizes into a columnar epithelial layer (top). Image courtesy of Jonathan Bowen.
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In This Issue
In Focus
Microtubules follow septins’ guidelines
Septin filaments direct the reorganization of microtubules during epithelial polarization.
People & Ideas
Rüdiger Klein: Reading the guideposts for axon guidance
Klein uses genetically modified mice to study how axons find the right targets to innervate.
Review
Report
APC/CCdh1-dependent proteolysis of USP1 regulates the response to UV-mediated DNA damage
APC/CCdh1-dependent degradation of USP1 allows for PCNA monoubiquitination and subsequent recruitment of trans-lesion synthesis polymerase to UV repair sites.
Septin GTPases spatially guide microtubule organization and plus end dynamics in polarizing epithelia
Filamentous and microtubule-associated septin GTPases guide the reorganization of the microtubule network during epithelial cell polarization.
Repeated cleavage failure does not establish centrosome amplification in untransformed human cells
Transient cleavage failure in dividing cells is not sufficient to establish stable populations of cells with extra centrosomes.
Article
Nuclear translocation of AMPK-α1 potentiates striatal neurodegeneration in Huntington’s disease
Nuclear accumulation of AMPK-α1 induces brain atrophy, neuronal loss, and formation of Htt aggregates in a Huntington’s disease model.
HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochore
The histone chaperone HJURP is a chromatin assembly factor that recruits CENP-A nucleosomes to centromeric chromatin.
Preferential binding of a kinesin-1 motor to GTP-tubulin–rich microtubules underlies polarized vesicle transport
The high affinity of KIF5 for microtubules rich in GTP-tubulin results in polarized motor protein accumulation at axonal tips in neurons and may underlie polarized vesicle transport.
High-resolution mapping reveals topologically distinct cellular pools of phosphatidylserine
Phosphatidylserine exists lumenally in the ER, Golgi, and mitochondria but cytoplasmically in the trans-Golgi and at the plasma membrane, which suggests that functionally important flipping may occur during trafficking.
A cell-autonomous requirement for neutral sphingomyelinase 2 in bone mineralization
nSMase2, which cleaves sphingomyelin to generate bioactive lipids, is required for chondrocyte apoptosis and, cell autonomously, for bone mineralization.
Competitive binding of Rab21 and p120RasGAP to integrins regulates receptor traffic and migration
P120RasGAP competes with Rab21 for binding to the cytoplasmic domain of integrin α-subunits, thereby promoting receptor escape from early endosomes and recycling to the plasma membrane.
Osteoblast mineralization requires β1 integrin/ICAP-1–dependent fibronectin deposition
ICAP-1 prevents recruitment of kindlin-2 to β1 integrin to control dynamics of fibrillar adhesion sites, fibronectin deposition, and osteoblast mineralization during bone formation.
Neuroligins/LRRTMs prevent activity- and Ca2+/calmodulin-dependent synapse elimination in cultured neurons
Neuroligins and leucine-rich repeat transmembrane proteins are necessary to prevent activity-dependent elimination of excitatory synapses in cultured neurons, with synapse elimination operating by a Ca2+/calmodulin-dependent pathway.
STIM1L is a new actin-binding splice variant involved in fast repetitive Ca2+ release
A newly identified splice variant of STIM1 called STIM1L forms constitutive clusters that interact with actin and Orai1 and allows fast repetitive Ca2+ release.
