-
Cover Image
Cover Image
On the cover
Uehara and Goshima follow the regrowth of depolymerized microtubules (green) in anaphase cells also stained for γ-tubulin (red) and DNA (blue). The Augmin complex is required for the de novo formation of anaphase microtubules and generation of a functional central spindle.
See page 259. - PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkEditorial Board
In This Issue
In Focus
A little Cdc20 goes a long way
Hypomorphic mice reveal new details of Cdc20 and cyclin B1's mitotic activities.
People & Ideas
Jiahuai Han: Aflame on inflammation and p38
Han studies the mechanisms of inflammation and immunity, and the many cellular functions of p38.
Editorial
Friends, colleagues, authors, lend us your data
The JCB was founded by a group of scientists who needed a journal to showcase their micrographs with the highest quality reproduction. Our commitment to innovative presentation and sharing of image data continues today with the JCB DataViewer.
Review
Report
RAD18-mediated ubiquitination of PCNA activates the Fanconi anemia DNA repair network
The DNA damage–activated E3 ubiquitin ligase RAD18 promotes repair of interstrand DNA cross-links by ubiquitylating PCNA and recruiting FANCL to chromatin.
Functional central spindle assembly requires de novo microtubule generation in the interchromosomal region during anaphase
The augmin protein complex nucleates noncentrosomal microtubules during anaphase to promote completion of cell division.
Aurora B kinase controls the targeting of the Astrin–SKAP complex to bioriented kinetochores
Localization of the spindle and kinetochore proteins Astrin, SKAP, and LC8 is antagonized by Aurora B so that they target exclusively to bioriented kinetochores.
Release of Mps1 from kinetochores is crucial for timely anaphase onset
Mps1 regulates its own turnover at kinetochores to ensure mitotic checkpoint silencing in metaphase.
Rescue of myogenic defects in Rb-deficient cells by inhibition of autophagy or by hypoxia-induced glycolytic shift
pRb is required to inhibit apoptosis in myoblasts and autophagy in myotubes but not for activation of the myogenic differentiation program.
Cell shape and contractility regulate ciliogenesis in cell cycle–arrested cells
Adhesive micropatterns show the effect of spatial confinement and actin network architecture on basal body positioning and primary cilium formation.
Article
Cdc20 hypomorphic mice fail to counteract de novo synthesis of cyclin B1 in mitosis
Low expression levels of Cdc20 result in chromatin bridging and chromosome misalignment, revealing a requirement for Cdc20 in efficient sister chromosome separation and chromosome–microtubule attachment.
Cep120 is asymmetrically localized to the daughter centriole and is essential for centriole assembly
Cep120, a protein involved in maintenance of neural progenitor cells, is required for centriole duplication in cycling cells and for centriole amplification in tracheal epithelial cells.
MyoD regulates apoptosis of myoblasts through microRNA-mediated down-regulation of Pax3
Suppression of the myogenic transcription factor MyoD is required for maintenance of muscle stem cells.
Family-wide characterization of the DENN domain Rab GDP-GTP exchange factors
Target or substrate Rab GTPases are identified for 17 proteins with DENN domains.
Ankyrin G restricts ion channel diffusion at the axonal initial segment before the establishment of the diffusion barrier
Ion channel immobilization by ankyrin G is regulated by casein kinase 2 in immature hippocampal neurons.
The scaffolding protein EBP50 regulates microvillar assembly in a phosphorylation-dependent manner
Both Cdc2 and PKC-mediated phosphorylation of EBP50 alter accessibility of its first PDZ domain when its second PDZ domain is occupied to regulate microvilli formation on epithelial cells.
A mechanism for vertebrate Hedgehog signaling: recruitment to cilia and dissociation of SuFu–Gli protein complexes
Hedgehog signaling through the ciliary membrane protein Smoothened dissociates the Gli transcription factors from their inhibitor, Suppressor of Fused.
