Using monoclonal antibodies as probes, we have characterized three antigens with respect to localization in the nucleolus, molecular weight and solubility. Two proteins, of 110,000 and 94,000 apparent molecular weight, were found associated with the ribonucleoprotein fibers. A third protein, with a molecular weight of 40,000, was accumulated at the nucleolar periphery, was present in the nucleoplasm, and may be involved in pre-ribosome maturation and transport.
Article| December 01 1984
Mapping nucleolar proteins with monoclonal antibodies.
U K Laemmli
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1984) 99 (6): 1981–1988.
J Kistler, Y Duncombe, U K Laemmli; Mapping nucleolar proteins with monoclonal antibodies.. J Cell Biol 1 December 1984; 99 (6): 1981–1988. doi: https://doi.org/10.1083/jcb.99.6.1981
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