We report here an ATP-dependent formation and contraction, or syneresis, of a microtubule gel using microtubule proteins prepared from calf brains. Gel contraction is typically observable 15-30 min after ATP addition to microtubules assembled to steady state, and is complete after approximately 60 min, at which time the gel volume is reduced by as much as 75%. In contracted gels, microtubule bundles and aster-like structures are observable. Gelation-contraction requires only microtubule proteins present after purification by three cycles of assembly and disassembly.
Article| October 01 1984
ATP-induced gelation--contraction of microtubules assembled in vitro.
R C Weisenberg
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1984) 99 (4): 1527–1533.
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R C Weisenberg, C Cianci; ATP-induced gelation--contraction of microtubules assembled in vitro.. J Cell Biol 1 October 1984; 99 (4): 1527–1533. doi: https://doi.org/10.1083/jcb.99.4.1527
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