The ability of glycopeptides, isolated from bovine cerebral cortex, to alter cell division was studied by cell-cycle analyses. The results showed that glycopeptides arrested baby hamster kidney (BHK)-21 cells and Chinese hamster ovary (CHO) cells in the G2 phase of the cell cycle. Upon removal of the growth inhibition from arrested BHK-21 cells, the mitotic index in colchicine-treated cultures increased from 5 to 40% within 6 h and the increase in mitotic activity was accompanied by a complete doubling of all arrested cells within this 6-h time period. Determination of DNA content in growth-arrested BHK-21 cells showed that growth-arrested cells contained about twice the DNA of control cell cultures. Although CHO cells treated in a like manner with growth inhibitor could not be arrested for the same length of time as BHK-21 cells (18 h vs. 72 h before initiation of escape) and to the same degree (60% of the cell population vs. 99% of BHK-21 cells), the escape kinetics of CHO cells did indicate a G2 arrest. Approximately 3.5 h after escape began, CHO cell numbers in treated cultures attained the cell numbers found in control cultures. This rapid growth phase occurring in less than 4 h indicated that the growth inhibitor induced a G2 arrest-point in CHO cells that was not lethal since the entire arrested cell population divided.
Article| August 01 1983
G2 cell cycle arrest induced by glycopeptides isolated from the bovine cerebral cortex.
P A Charp
R J Kinders
T C Johnson
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1983) 97 (2): 311–316.
P A Charp, R J Kinders, T C Johnson; G2 cell cycle arrest induced by glycopeptides isolated from the bovine cerebral cortex.. J Cell Biol 1 August 1983; 97 (2): 311–316. doi: https://doi.org/10.1083/jcb.97.2.311
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