A new two-dimensional ribosome crystal, having the tetragonal space group P42(1)2 (a = 593 A), has been grown from ribosome tetramers extracted from hypothermic chick embryos. It is of particular interest because of its larger size (up to 3 x 3 micron2) and greater stability compared to other related polymorphic forms, and because it can easily be grown in large amounts. X-ray diffraction shows the order in the crystal to extend to a resolution of at least 60 A. The crystalline ribosomes appear to contain a full complement of small and large ribosomal subunit proteins and an additional four proteins not characteristic of chick embryo polysomes.
Article| November 01 1982
In vitro crystallization of ribosomes from chick embryos.
R A Milligan
P N Unwin
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1982) 95 (2): 648–653.
R A Milligan, P N Unwin; In vitro crystallization of ribosomes from chick embryos.. J Cell Biol 1 November 1982; 95 (2): 648–653. doi: https://doi.org/10.1083/jcb.95.2.648
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