The pattern and schedule of histone synthesis in unfertilized eggs and early embryos of the sea urchin Strongylocentrotus purpuratus were studied using two-dimensional gel electrophoresis. After fertilization there is an abrupt change in the pattern of histone variant synthesis. Although both cleavage-stage (CS) variants. However, after fertilization, both CS and alpha messages are translated. Since alpha histone mRNA isolated from unfertilized eggs can be translated in vitro, the synthesis of alpha histone subtypes appears to be under translational control. Although the synthesis of alpha subtypes is shown here to occur before the second S phase after fertilization, little or no alpha histone is incorporated into chromatin at this time. Thus, early chromatin is composed predominantly of CS variants probably recruited for the most part from the large pool of CS histones stored in the unfertilized egg.
Article| July 01 1982
Translational regulation of histone synthesis in the sea urchin strongylocentrotus purpuratus.
V G Allfrey
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1982) 94 (1): 219–223.
L Herlands, V G Allfrey, D Poccia; Translational regulation of histone synthesis in the sea urchin strongylocentrotus purpuratus.. J Cell Biol 1 July 1982; 94 (1): 219–223. doi: https://doi.org/10.1083/jcb.94.1.219
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