To monitor the interaction of cell surface acetylcholine (AcCho) receptors with the cytoskeleton, cultured muscle cells were labeled with radioactive or fluorescent alpha-bungarotoxin and extracted with Triton X-100, using conditions that preserve internal structure. A significant population of the AcCho receptors is retained on the skeletal framework remaining after detergent extraction. The skeleton organization responsible for restricting AcCho receptors to a patched region may also result in their retention after detergent extraction.
Interaction of the cytoskeletal framework with acetylcholine receptor on th surface of embryonic muscle cells in culture.
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J Prives, A B Fulton, S Penman, M P Daniels, C N Christian; Interaction of the cytoskeletal framework with acetylcholine receptor on th surface of embryonic muscle cells in culture.. J Cell Biol 1 January 1982; 92 (1): 231–236. doi: https://doi.org/10.1083/jcb.92.1.231
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