The effects of epidermal growth factor (EGF) on the cytokeratin filaments of cultured murine epithelial cells were studied by the indirect immunofluorescence technique with affinity-purified antibodies. Mouse epithelial cells (MMC-E), grown on glass cover slips, and viewed by immunofluorescence microscopy, showed keratin-specific fluorescence as typical bright perinuclear aggregates corresponding to dense paracrystalline granules seen in electron microscopy. Within minutes after an exposure to EGF, the keratin granules in the MMC-E cells decreased. After 10 min of incubation, the cells had spread fibrillar keratin. Such an effect could not be found after a similar exposure to insulin, dexamethasone, dibutyryl cyclic AMP, or antimitotic drugs. EGF, therefore, has a relatively direct effect on the cytoskeletal organization of cultured epithelial cells. These rapid effects on the keratin filaments may explain the simultaneous EGF-induced ultrastructural surface changes of the cells. EGF may thus function as a regulatory factor in the migration of epithelial cells and in the mobility of their cell membranes. The epithelial cell line, MMC-E, should prove a useful model for studies on the action of EGF on nontransformed epithelial cells in vitro.
Article| August 01 1981
Keratin filaments of mouse epithelial cells are rapidly affected by epidermal growth factor.
V P Lehto
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1981) 90 (2): 537–541.
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J Keski-Oja, V P Lehto, I Virtanen; Keratin filaments of mouse epithelial cells are rapidly affected by epidermal growth factor.. J Cell Biol 1 August 1981; 90 (2): 537–541. doi: https://doi.org/10.1083/jcb.90.2.537
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