The distribution of tubulin in soluble, reversibly stabilized (assembled) and insoluble forms has been determined in neuroblastoma cells undergoing microtubule-dependent neurite elongation. Procedures were developed to obtain reproducible tubulin fractions and to assay total tubulin. Radioimmunoassays showed that both differentiated and nondifferentiated cell contained approximately 4 pg of tubulin per cell, of which 3-10% was in an insoluble, particulate form. The amount of tubulin assembled in differentiated cells was four to five times greater than in nondifferentiated cells, constituting 48-63% and 11-16% of the total tubulin pool in the respective cell types. Calculation of the concentration of soluble tubulin indifferentiated cells (approximately 0.8 mg/ml) and nondifferentiated cells (approximately 1.6 mg/ml) indicates that a critical concentration of subunits probably does not limit the induction of microtubule formation during neurite elongation.
Article| June 01 1981
Tubulin pools in differentiating neuroblastoma cells.
J B Olmsted
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1981) 89 (3): 418–423.
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J B Olmsted; Tubulin pools in differentiating neuroblastoma cells.. J Cell Biol 1 June 1981; 89 (3): 418–423. doi: https://doi.org/10.1083/jcb.89.3.418
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