A kinetic analysis of the appearance of [3H]uridine label in RNA sequences that neighbor poly(A), as well as the incorporation of [3H]adenosine label into both the RNA chain and the poly(A) of poly(A)-containing molecules, shows that poly(A) is added within a minute or so after RNA chain synthesis in Chinese hamster ovary cells and HeLa cells. Previous conclusions by several groups (5-7) that poly(A) might be added as long as 20-30 min after RNA synthesis appear to be in error, and the present conclusion seems much more in line with several different types of recent studies with specific mRNAs that suggest prompt poly(A) addition (13-16).
Article| September 01 1980
Addition of poly(A) to nuclear RNA occurs soon after RNA synthesis.
J E Darnell, Jr
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1980) 86 (3): 844–848.
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M Salditt-Georgieff, M Harpold, S Sawicki, J Nevins, J E Darnell; Addition of poly(A) to nuclear RNA occurs soon after RNA synthesis.. J Cell Biol 1 September 1980; 86 (3): 844–848. doi: https://doi.org/10.1083/jcb.86.3.844
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