The requirement of cholesterol for myoblast fusion has been linked to the primary step in the fusion process, calcium-dependent aggregation (recognition). Inhibition of cholesterol synthesis with 25-hydroxycholesterol or compactin in the absence of exogenous lipid dramatically inhibits calcium-mediated aggregation and concomitant fusion within several hours. Restimulating cholesterol synthesis or supplying exogenous cholesterol rapidly restores aggregation activity. Over this time period, however, the sterol:phospholipid ratio is unaltered, suggesting a local rather than a general membrane cholesterol requirement for the expression of aggregation activity. The aggregation response to a change in sterol availability occurs on a shorter time scale than that required to inhibit the synthesis of the protein(s) with aggregation activity; thus, the cholesterol-requiring step is posttranslational. We suggest that the assembly or maintenance of the aggregation activity depends on a continued local supply of cholesterol.
Article| September 01 1980
Cholesterol availability modulates myoblast fusion.
R B Cornell
S M Nissley
A F Horwitz
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1980) 86 (3): 820–824.
R B Cornell, S M Nissley, A F Horwitz; Cholesterol availability modulates myoblast fusion.. J Cell Biol 1 September 1980; 86 (3): 820–824. doi: https://doi.org/10.1083/jcb.86.3.820
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