Quantitative and qualitative changes in cellular actin were followed during differentiation of a myeloid leukemia cell line, namely Ml, which was inducible with conditioned medium (CM). During 3 d of incubation with CM, when the Ml cells differentiated to macrophages and lost their mitotic activity, the actin content, F-actin ratio in total actin, and the actin synthesis showed an increase. A greater difference before and after differentiation was found in the ability of G-actin to polymerize. Actin harvested from CM-treated cells showed a greater ability to polymerize, depending on the increased concentration of MgCl2 and/or KCl and proteins, as compared with the actin from untreated Ml cells. Actin harvested from the Mml cell line, a macrophage line, had a particularly high polymerizability with or without CM treatment. In contrast, the actin from the D- subline, which is insensitive to CM, showed almost no polymerization.
Article| May 01 1980
Changes in contractile proteins during differentiation of myeloid leukemia cells. I. Polymerization of actin.
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1980) 85 (2): 273–282.
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K Nagata, J Sagara, Y Ichikawa; Changes in contractile proteins during differentiation of myeloid leukemia cells. I. Polymerization of actin.. J Cell Biol 1 May 1980; 85 (2): 273–282. doi: https://doi.org/10.1083/jcb.85.2.273
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