A new indirect method for fluorescence localization of proteins making use of the avidin-biotin complex is described. We have prepared both a biotin-modified rabbit heavy meromyosin (BHMM) and a biotin-modified antibody to a smooth muscle myosin. After fixation, cells can be treated with either BHMM, which binds to actin, or the biotinyl antibody, which binds to myosin. In a second step the cell are treated with a fluorescent derivative of avidin (Fl-avidin) which binds to the biotinyl proteins and thus indirectly reveals the location of the cellular action or myosin.
Article| June 01 1977
Use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy.
M H Heggeness
J F Ash
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1977) 73 (3): 783–788.
M H Heggeness, J F Ash; Use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy.. J Cell Biol 1 June 1977; 73 (3): 783–788. doi: https://doi.org/10.1083/jcb.73.3.783
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