To study the effect of EDTA on cell wall structure and the reversal of this effect by uranyl ion, thin sections of pea root tips were examined in the electron microscope. EDTA is known to facilitate separation of the cells in root tips. When sections of fixed and embedded EDTA-treated roots are floated on a uranyl-acetate solution, a loose network is revealed that would seem to be cellulose. Incorporation of uranyl into the roots, if it occurs prior to fixation, brings about recementation of the cells. After such treatment, a marginal darker area and a median brighter one can be observed in the wall, and the whole structure appears more compact again. Comparison of the results of the various treatments suggests that cellulose-cementing material is dispersed throughout the entire wall, and that its distribution parallels that of cellulose.
Article|
April 01 1960
An Electron Microscopic Investigation into the Effect of EDTA on Plant Cell Wall
Shimon Klein,
Shimon Klein
From the Department of Botany, The Hebrew University, Jerusalem
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B. Ginzburg
B. Ginzburg
From the Department of Botany, The Hebrew University, Jerusalem
Search for other works by this author on:
Shimon Klein
From the Department of Botany, The Hebrew University, Jerusalem
B. Ginzburg
From the Department of Botany, The Hebrew University, Jerusalem
Received:
May 20 1959
Copyright, 1960, by The Rockefeller Institute Press
1960
J Biophys and Biochem Cytol (1960) 7 (2): 335–338.
Article history
Received:
May 20 1959
Citation
Shimon Klein, B. Ginzburg; An Electron Microscopic Investigation into the Effect of EDTA on Plant Cell Wall . J Biophys and Biochem Cytol 1 April 1960; 7 (2): 335–338. doi: https://doi.org/10.1083/jcb.7.2.335
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