Fibroblast growth factor (FGF), a polypeptide that has been shown to stimulate division in 3T3 cells, was tested for mitogenic effects on diploid, early-passage cells from human and murine sources. The quantitative assay of [3H]thymidine incorporation into acid-insoluble material showed that FGF at low concentrations (10 minus 9 M) was more effective than additional serum for provoking the initiation of DNA synthesis in human foreskin fibroblasts or mouse fibroblasts maintained in 5 or 10% serum, respectively. The growth of the human fibroblasts was twice as fast in the presence of FGF plus 10% calf serum as it was in the presence of 10% calf serum or 20% fetal calf serum alone. The addition of FGF to primary cultures of mouse fibroblasts in 0.4% serum resulted in a twofold increase in cell number compared to controls. In contrast to results obtained with 3T3 cells, neither insulin nor a glucocorticoid potentiated the effects of FGF on either human or mouse cells.
Article| August 01 1975
Mitogenic effect of fibroblast growth factor on early passage cultures of human and murine fibroblasts.
J S Moran
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1975) 66 (2): 451–457.
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D Gospodarowicz, J S Moran; Mitogenic effect of fibroblast growth factor on early passage cultures of human and murine fibroblasts.. J Cell Biol 1 August 1975; 66 (2): 451–457. doi: https://doi.org/10.1083/jcb.66.2.451
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