The localization of concanavalin A (Con A) binding sites in Purkinje cell somata and dendrites has been studied using a peroxidase labeling technique. In the somata, the nuclear, Golgi, and endoplasmic reticulum (ER) membranes are rich in Con A binding sites. The hypolemmal cisternae, which are continuous with the ER from the soma and throughout the dendritic tree of Purkinje cells, are also rich in Con A binding sites. Other cisternae seen in these dendrites do not bind detectable amounts of Con A. The results suggest that a cisternal system, rich in carbohydrate, may be continuous from the nuclear envelope to distal dendritic segments of Purkinje cells. Such a system could play a role in the movement of materials from Purkinje somata to dendrites.
THE VISUALIZATION OF CONCANAVALIN A BINDING SITES IN PURKINJE CELL SOMATA AND DENDRITES OF RAT CEREBELLUM
John G. Wood, Barbara J. McLaughlin, Robert P. Barber; THE VISUALIZATION OF CONCANAVALIN A BINDING SITES IN PURKINJE CELL SOMATA AND DENDRITES OF RAT CEREBELLUM . J Cell Biol 1 November 1974; 63 (2): 541–549. doi: https://doi.org/10.1083/jcb.63.2.541
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