Giant axons from the squid, Loligo pealei, were fixed in glutaraldehyde and postfixed in osmium tetroxide. Calcium chloride (5 mM/liter) was added to all aqueous solutions used for tissue processing. Electron-opaque deposits were found along the axonal plasma membranes, within mitochondria, and along the basal plasma membranes of Schwann cells. X-ray microprobe analysis (EMMA-4) yielded signals for calcium and phosphorus when deposits were probed, whereas these elements were not detected in the axoplasm.
ASSOCIATION OF CALCIUM WITH MEMBRANES OF SQUID GIANT AXON : Ultrastructure and Microprobe Analysis
J. L. Oschman, T. A. Hall, P. D. Peters, B. J. Wall; ASSOCIATION OF CALCIUM WITH MEMBRANES OF SQUID GIANT AXON : Ultrastructure and Microprobe Analysis . J Cell Biol 1 April 1974; 61 (1): 156–165. doi: https://doi.org/10.1083/jcb.61.1.156
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