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Volume 56, Issue 1
1 January 1973
Journal of Cell Biology Cover Image for Volume 56, Issue 1
Article| January 01 1973

AN IMPROVED CELL FRACTIONATION PROCEDURE FOR THE PREPARATION OF RAT LIVER MEMBRANE-BOUND RIBOSOMES

M. R. Adelman,
M. R. Adelman
From The Rockefeller University, New York 10021
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Gunter Blobel,
Gunter Blobel
From The Rockefeller University, New York 10021
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David D. Sabatini
David D. Sabatini
From The Rockefeller University, New York 10021
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Author and Article Information
M. R. Adelman
From The Rockefeller University, New York 10021
Gunter Blobel
From The Rockefeller University, New York 10021
David D. Sabatini
From The Rockefeller University, New York 10021
Dr Adelman's present address is the Department of Anatomy, Duke University Medical Center, Durham, North Carolina 27710.
Received: July 10 1972
Revision Received: August 16 1972
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1973 by The Rockefeller University Press
1973
J Cell Biol (1973) 56 (1): 191–205.
https://doi.org/10.1083/jcb.56.1.191
Article history
Received:
July 10 1972
Revision Received:
August 16 1972

A cell fractionation procedure is described which allows the preparation from rat liver of a rough microsome population containing almost 50% of the membrane-bound ribosomes of the tissue. The fraction is not contaminated with free ribosomes or smooth microsomes, and, by various other criteria, is suitable for studies of ribosome-membrane interaction.

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Copyright © 1973 by The Rockefeller University Press
1973
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