Quantitative ultrastructural analysis and quantitative gel electrophoresis of preparations of selectively solubilized Chlamydomonas outer doublets indicated that tubulins 1 and 2 were present in both the A tubule and the B tubule, and that only tubulin 1 was present in the three protofilaments which form the wall ("partition") between the lumens of the A and B tubules. The data suggested that the remaining protofilaments of the outer doublet were grouped together in pairs containing the same type of tubulin, pairs containing tubulin 1 alternating with pairs containing tubulin 2. These findings were used to construct models for the arrangement of the two tubulins in the outer doublet. Further analysis by isoelectric focusing resolved tubulins 1 and 2 into at least five bands.
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September 01 1972
CHLAMYDOMONAS FLAGELLA : II. The Distribution of Tubulins 1 and 2 in the Outer Doublet Microtubules
G. B. Witman
,
G. B. Witman
From the Department of Biology, Yale University, New Haven, Connecticut 06520.
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K. Carlson
,
K. Carlson
From the Department of Biology, Yale University, New Haven, Connecticut 06520.
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Joel L. Rosenbaum
Joel L. Rosenbaum
From the Department of Biology, Yale University, New Haven, Connecticut 06520.
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G. B. Witman
From the Department of Biology, Yale University, New Haven, Connecticut 06520.
K. Carlson
From the Department of Biology, Yale University, New Haven, Connecticut 06520.
Joel L. Rosenbaum
From the Department of Biology, Yale University, New Haven, Connecticut 06520.
Dr. Witman's present address is Whitman Laboratory, Department of Biology, University of Chicago, Chicago, Illinois 60601.
Received:
March 06 1972
Revision Received:
May 05 1972
Online Issn: 1540-8140
Print Issn: 0021-9525
Copyright © 1972 by The Rockefeller University Press
1972
J Cell Biol (1972) 54 (3): 540–555.
Article history
Received:
March 06 1972
Revision Received:
May 05 1972
Citation
G. B. Witman, K. Carlson, Joel L. Rosenbaum; CHLAMYDOMONAS FLAGELLA : II. The Distribution of Tubulins 1 and 2 in the Outer Doublet Microtubules . J Cell Biol 1 September 1972; 54 (3): 540–555. doi: https://doi.org/10.1083/jcb.54.3.540
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