X-ray diffraction photographs of a nucleoprotein preparation from Escherichia coli show the A-type pattern of crystalline deoxyribonucleic acid (DNA). This suggests that a large part of the DNA is free from protein. In higher organisms DNA does not exist in this form but is closely bound to protein.
The Absence of Histone in the Bacterium Escherichia coli : II. X-ray Diffraction of Nucleoprotein Extract
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M. H. F. Wilkins, G. Zubay; The Absence of Histone in the Bacterium Escherichia coli : II. X-ray Diffraction of Nucleoprotein Extract . J Biophys and Biochem Cytol 25 January 1959; 5 (1): 55–58. doi: https://doi.org/10.1083/jcb.5.1.55
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