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Volume 47, Issue 2
1 November 1970
Journal of Cell Biology Cover Image for Volume 47, Issue 2
Article| November 01 1970

PURIFICATION OF INTACT MICROTUBULES FROM BRAIN

Joel B. Kirkpatrick,
Joel B. Kirkpatrick
From the Department of Pharmacology, Rutgers Medical School, New Brunswick, New Jersey 08903
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Lyon Hyams,
Lyon Hyams
From the Department of Pharmacology, Rutgers Medical School, New Brunswick, New Jersey 08903
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Virginia L. Thomas,
Virginia L. Thomas
From the Department of Pharmacology, Rutgers Medical School, New Brunswick, New Jersey 08903
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Peter M. Howley
Peter M. Howley
From the Department of Pharmacology, Rutgers Medical School, New Brunswick, New Jersey 08903
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Author and Article Information
Joel B. Kirkpatrick
From the Department of Pharmacology, Rutgers Medical School, New Brunswick, New Jersey 08903
Lyon Hyams
From the Department of Pharmacology, Rutgers Medical School, New Brunswick, New Jersey 08903
Virginia L. Thomas
From the Department of Pharmacology, Rutgers Medical School, New Brunswick, New Jersey 08903
Peter M. Howley
From the Department of Pharmacology, Rutgers Medical School, New Brunswick, New Jersey 08903
Received: February 03 1970
Revision Received: June 29 1970
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1970 by The Rockefeller University Press
1970
J Cell Biol (1970) 47 (2): 384–394.
https://doi.org/10.1083/jcb.47.2.384
Article history
Received:
February 03 1970
Revision Received:
June 29 1970

Hundred-fold purification of intact microtubules from homogenates of rat brain is reported. The success of purification depends on stabilizing the microtubule structure by the combined effects of hexylene glycol, acidic Ph, and low temperature. A practical, negative stain, electron microscopic assay is used to study purity and stability of microtubule fractions. The purified fractions show a major band which migrates like purified tubulin in the SDS gel electrophoresis system.

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Copyright © 1970 by The Rockefeller University Press
1970
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