Golgi apparatus were released without fixatives from rat hepatocytes by gentle homogenization, concentrated by differential centrifugation, and purified by sucrose gradient centrifugation. Examination of sections of purified fractions by electron microscopy showed fields of morphologically intact units of Golgi apparatus consisting of stacks of parallel flattened cisternae, secretory vesicles, and small vesicular profiles. Negative staining of unfixed pellets revealed a complex network of anastomotic tubules continuous with platelike structures and secretory vesicles. These structures corresponded, respectively, to the small vesicular profiles and parallel flattened cisternae with attached secretory vesicles of sectioned material. Small fragments of granular endoplasmic reticulum were often closely associated with the peripheral tubules, suggesting sites of continuity in intact hepatocytes.
Article|
March 01 1970
ISOLATION OF A GOLGI APPARATUS-RICH FRACTION FROM RAT LIVER : I. Method and Morphology
D. James Morré,
D. James Morré
From the Departments of Botany and Plant Pathology and Biology, Purdue University, Lafayette, Indiana 47907; Departments of Anatomy and Pathology, Vanderbilt University Medical School, Nashville, Tennessee 37203; Charles F. Kettering Research Laboratory, Yellow Springs, Ohio 45387; and the Department of Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55101
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R. L. Hamilton,
R. L. Hamilton
From the Departments of Botany and Plant Pathology and Biology, Purdue University, Lafayette, Indiana 47907; Departments of Anatomy and Pathology, Vanderbilt University Medical School, Nashville, Tennessee 37203; Charles F. Kettering Research Laboratory, Yellow Springs, Ohio 45387; and the Department of Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55101
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H. H. Mollenhauer,
H. H. Mollenhauer
From the Departments of Botany and Plant Pathology and Biology, Purdue University, Lafayette, Indiana 47907; Departments of Anatomy and Pathology, Vanderbilt University Medical School, Nashville, Tennessee 37203; Charles F. Kettering Research Laboratory, Yellow Springs, Ohio 45387; and the Department of Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55101
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R. W. Mahley,
R. W. Mahley
From the Departments of Botany and Plant Pathology and Biology, Purdue University, Lafayette, Indiana 47907; Departments of Anatomy and Pathology, Vanderbilt University Medical School, Nashville, Tennessee 37203; Charles F. Kettering Research Laboratory, Yellow Springs, Ohio 45387; and the Department of Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55101
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W. P. Cunningham,
W. P. Cunningham
From the Departments of Botany and Plant Pathology and Biology, Purdue University, Lafayette, Indiana 47907; Departments of Anatomy and Pathology, Vanderbilt University Medical School, Nashville, Tennessee 37203; Charles F. Kettering Research Laboratory, Yellow Springs, Ohio 45387; and the Department of Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55101
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R. D. Cheetham,
R. D. Cheetham
From the Departments of Botany and Plant Pathology and Biology, Purdue University, Lafayette, Indiana 47907; Departments of Anatomy and Pathology, Vanderbilt University Medical School, Nashville, Tennessee 37203; Charles F. Kettering Research Laboratory, Yellow Springs, Ohio 45387; and the Department of Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55101
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V. S. Lequire
V. S. Lequire
From the Departments of Botany and Plant Pathology and Biology, Purdue University, Lafayette, Indiana 47907; Departments of Anatomy and Pathology, Vanderbilt University Medical School, Nashville, Tennessee 37203; Charles F. Kettering Research Laboratory, Yellow Springs, Ohio 45387; and the Department of Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55101
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D. James Morré
From the Departments of Botany and Plant Pathology and Biology, Purdue University, Lafayette, Indiana 47907; Departments of Anatomy and Pathology, Vanderbilt University Medical School, Nashville, Tennessee 37203; Charles F. Kettering Research Laboratory, Yellow Springs, Ohio 45387; and the Department of Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55101
R. L. Hamilton
From the Departments of Botany and Plant Pathology and Biology, Purdue University, Lafayette, Indiana 47907; Departments of Anatomy and Pathology, Vanderbilt University Medical School, Nashville, Tennessee 37203; Charles F. Kettering Research Laboratory, Yellow Springs, Ohio 45387; and the Department of Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55101
H. H. Mollenhauer
From the Departments of Botany and Plant Pathology and Biology, Purdue University, Lafayette, Indiana 47907; Departments of Anatomy and Pathology, Vanderbilt University Medical School, Nashville, Tennessee 37203; Charles F. Kettering Research Laboratory, Yellow Springs, Ohio 45387; and the Department of Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55101
R. W. Mahley
From the Departments of Botany and Plant Pathology and Biology, Purdue University, Lafayette, Indiana 47907; Departments of Anatomy and Pathology, Vanderbilt University Medical School, Nashville, Tennessee 37203; Charles F. Kettering Research Laboratory, Yellow Springs, Ohio 45387; and the Department of Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55101
W. P. Cunningham
From the Departments of Botany and Plant Pathology and Biology, Purdue University, Lafayette, Indiana 47907; Departments of Anatomy and Pathology, Vanderbilt University Medical School, Nashville, Tennessee 37203; Charles F. Kettering Research Laboratory, Yellow Springs, Ohio 45387; and the Department of Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55101
R. D. Cheetham
From the Departments of Botany and Plant Pathology and Biology, Purdue University, Lafayette, Indiana 47907; Departments of Anatomy and Pathology, Vanderbilt University Medical School, Nashville, Tennessee 37203; Charles F. Kettering Research Laboratory, Yellow Springs, Ohio 45387; and the Department of Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55101
V. S. Lequire
From the Departments of Botany and Plant Pathology and Biology, Purdue University, Lafayette, Indiana 47907; Departments of Anatomy and Pathology, Vanderbilt University Medical School, Nashville, Tennessee 37203; Charles F. Kettering Research Laboratory, Yellow Springs, Ohio 45387; and the Department of Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55101
Received:
May 08 1969
Revision Received:
September 09 1969
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1970 by The Rockefeller University Press
1970
J Cell Biol (1970) 44 (3): 484–491.
Article history
Received:
May 08 1969
Revision Received:
September 09 1969
Citation
D. James Morré, R. L. Hamilton, H. H. Mollenhauer, R. W. Mahley, W. P. Cunningham, R. D. Cheetham, V. S. Lequire; ISOLATION OF A GOLGI APPARATUS-RICH FRACTION FROM RAT LIVER : I. Method and Morphology . J Cell Biol 1 March 1970; 44 (3): 484–491. doi: https://doi.org/10.1083/jcb.44.3.484
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