Fertilized, but still unsegmented, eggs of the sea urchin Paracentrotus lividus have been centrifugated at 30,000 gravity. A comparison has been made between the cytochemical reactions of the different layers (RNA, polysaccharides, acid phosphatase) and the fine structure as shown in thin sections studied with the electron microscope.
Cytochemical Localisations and Ultrastructure in the Fertilized Unsegmented Egg of Paracentrotus lividus
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J. J. Pasteels, P. Castiaux, G. Vandermeerssche; Cytochemical Localisations and Ultrastructure in the Fertilized Unsegmented Egg of Paracentrotus lividus . J Biophys and Biochem Cytol 25 September 1958; 4 (5): 575–578. doi: https://doi.org/10.1083/jcb.4.5.575
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