Colloidal gold was injected into the cytoplasm of amebae (Amoeba proteus) approximately 5 min, 1 hr, and 2 hr after cytokinesis. Later, interphase cells were similarly treated. All of the amebae were fixed about 50 min after injection and were examined with the electron microscope in order to determine the distribution of the gold. It was found that for a period of 2 hr after division the uptake of gold by the nuclei was significantly greater than that during late interphase. Correlation of the gold distribution with the morphology of the nuclear envelope indicated that an inverse relationship exists between the rate of incorporation of colloid into the nucleoplasm and the degree of reconstitution of the fibrous lamina ("honeycomb" structure). These data support the view that the fibrous lamina functions in regulating nucleocytoplasmic exchanges.
Article| October 01 1968
NUCLEOCYTOPLASMIC EXCHANGES DURING EARLY INTERPHASE
Carl M. Feldherr
From the Department of Anatomical Sciences, the University of Florida, Gainesville, Florida 32601
Received: February 12 1968
Online Issn: 1540-8140
Print Issn: 0021-9525
Copyright © 1968 by The Rockefeller University Press.
- Views Icon Views
- PDF LinkPDF
- Share Icon Share
- Search Site
Carl M. Feldherr; NUCLEOCYTOPLASMIC EXCHANGES DURING EARLY INTERPHASE . J Cell Biol 1 October 1968; 39 (1): 49–54. doi: https://doi.org/10.1083/jcb.39.1.49
Download citation file: