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Volume 28, Issue 3
1 March 1966
Journal of Cell Biology Cover Image for Volume 28, Issue 3
Article| March 01 1966

STUDIES ON INSULIN BIOSYNTHESIS : Subcellular Distribution of Leucine-H3 Radioactivity During Incubation of Goosefish Islet Tissue

G. Eric Bauer,
G. Eric Bauer
From the Department of Anatomy, University of Minnesota, Minneapolis, and the Marine Biological Laboratory, Woods Hole, Massachusetts
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Arnold W. Lindall, Jr.,
Arnold W. Lindall, Jr.
From the Department of Anatomy, University of Minnesota, Minneapolis, and the Marine Biological Laboratory, Woods Hole, Massachusetts
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Padmaker K. Dixit,
Padmaker K. Dixit
From the Department of Anatomy, University of Minnesota, Minneapolis, and the Marine Biological Laboratory, Woods Hole, Massachusetts
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Gordon Lester,
Gordon Lester
From the Department of Anatomy, University of Minnesota, Minneapolis, and the Marine Biological Laboratory, Woods Hole, Massachusetts
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Arnold Lazarow
Arnold Lazarow
From the Department of Anatomy, University of Minnesota, Minneapolis, and the Marine Biological Laboratory, Woods Hole, Massachusetts
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Author and Article Information
G. Eric Bauer
From the Department of Anatomy, University of Minnesota, Minneapolis, and the Marine Biological Laboratory, Woods Hole, Massachusetts
Arnold W. Lindall, Jr.
From the Department of Anatomy, University of Minnesota, Minneapolis, and the Marine Biological Laboratory, Woods Hole, Massachusetts
Padmaker K. Dixit
From the Department of Anatomy, University of Minnesota, Minneapolis, and the Marine Biological Laboratory, Woods Hole, Massachusetts
Gordon Lester
From the Department of Anatomy, University of Minnesota, Minneapolis, and the Marine Biological Laboratory, Woods Hole, Massachusetts
Arnold Lazarow
From the Department of Anatomy, University of Minnesota, Minneapolis, and the Marine Biological Laboratory, Woods Hole, Massachusetts
Received: September 03 1965
Online ISSN: 1540-8140
Print ISSN: 0021-9525
1966
J Cell Biol (1966) 28 (3): 413–421.
https://doi.org/10.1083/jcb.28.3.413
Article history
Received:
September 03 1965

Islet tissue from the goosefish (Lophius piscatorius) was incubated in the presence of leucine-H3. The tissue was then separated into subcellular fractions and the radioactivity determined in total acid alcohol-soluble proteins, insoluble proteins, and insulin. At any time, microsomal protein had a higher activity than secretion granule protein. Pulse-chase experiments further suggest the microsomes as primary sites of protein synthesis. The data are evidence for microsomal synthesis of insulin and for its subsequent transfer into the secretion granules.

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1966
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