Bovine retinal outer segment fragments were isolated by density gradient centrifugation in a high centrifugal field. Assays of the final preparation for enzymes of the mitochondrial respiratory chain indicated mitochondrial contamination not in excess of 1 per cent. Glucose-6-phosphatase and TPNH-cytochrome c reductase activities, presumably diagnostic for microsomes, were also absent. Electron micrographs did not disclose the presence of significant numbers of particles other than fragments of the outer segment discs. The red fragments were characterized by an ascorbate-oxidizing system and a high lipid content.
Article| December 01 1965
THE ISOLATION OF RETINAL OUTER SEGMENT FRAGMENTS
David G. McConnell
From the Institute for Enzyme Research, University of Wisconsin, Madison.
Dr. McConnell's permanent address is the Institute for Research in Vision, the Ohio State University, Columbus
Received: January 27 1965
Online ISSN: 1540-8140
Print ISSN: 0021-9525
David G. McConnell; THE ISOLATION OF RETINAL OUTER SEGMENT FRAGMENTS . J Cell Biol 1 December 1965; 27 (3): 459–473. doi: https://doi.org/10.1083/jcb.27.3.459
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