page 201, demonstrating that Mdc1/NFBD1 precedes 53BP1's arrival and is required for 53BP1's stable association with the chromatin surrounding DSBs.
Mdc1 and 53BP1 are two of the earliest proteins to accumulate at DSBs, but recent studies reported conflicting data as to how the proteins influence each other's binding at damaged chromosomes. To find out, Bekker-Jensen et al. followed the assembly process in real time. They used a micro-laser to generate DSBs in tissue culture cells and started time-lapse imaging immediately.
GFP-tagged 53BP1 and Mdc1 began to accumulate at the sites of double strand breaks within minutes of damage. As expected, 53BP1 protein that lacked the Tudor domain, which is required for interaction with dimethylated histone H3 (H3-dmK79), did not aggregate at the breaks. Simultaneous imaging of a mixed culture of cells expressing labeled 53BP1 or Mdc1 showed that Mdc1 arrived at DNA breaks before 53BP1. In cells depleted of Mdc1 by siRNA treatment, much less 53BP1 bound to the DNA breaks. The 53BP1 protein that did bind drifted away more rapidly than in cells with Mdc1, likely due to an absence of phosphorylation of histone H2AX (γ-H2AX), which was previously shown to be important for 53BP1 binding. In the reciprocal experiment, Mdc1 binding was unaffected by 53BP1 siRNA depletion.Bekker-Jensen et al. propose that after the initial sensor, the Mre11-Nbs1-Rad50 complex, detects a double strand break, ATM launches a phosphorylation cascade, including phosphorylation of histone H2AX. Mdc1 binds to γ-H2AX, altering the chromatin structure and unmasking H3-dmK79, a constitutive histone modification. 53BP1 forms a stable interaction with the exposed H3-dmK79 and settles in at the damage. The stepwise building of the Mdc1-53BP1 platform includes changes in local chromatin architecture around the DSBs and is probably important for increasing the local concentration of repair proteins.