Observations have been made on the response, in vitro, of cultured and freshly dissociated cells to mechanical deformation. Large numbers of individual cells were studied by means of a special culture chamber bounded by two parallel glass coverslips whose spacing could be reduced from 140 to 2 microns in steps of roughly 0.5 micron. The degree of deformation required for herniation of the cell surface was measured. These measurements lead to the definition of a statistical index characteristic of the extensibility of cell surfaces. This index has been shown to be distinctive for several types of cells; to alter with certain stages of embryonic development; and to be stable with respect to the culturing of cells and certain alterations in the method of cell culture.
Article|
May 01 1963
THE RELATIVE EXTENSIBILITY OF CELL SURFACES
Murray D. Rosenberg
Murray D. Rosenberg
From The Rockefeller Institute
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Murray D. Rosenberg
From The Rockefeller Institute
Received:
July 27 1962
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright, 1963, by The Rockefeller Institute Press
1963
J Cell Biol (1963) 17 (2): 289–297.
Article history
Received:
July 27 1962
Citation
Murray D. Rosenberg; THE RELATIVE EXTENSIBILITY OF CELL SURFACES . J Cell Biol 1 May 1963; 17 (2): 289–297. doi: https://doi.org/10.1083/jcb.17.2.289
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