Plane through a crystal of tobacco ringspot virus (left) made using Steere's do-it-yourself cold operating box (middle). A later freeze fracture image is shown at right.
STEERE/STAEHELIN
With this modest claim, Steere et al. (1957) introduced a method that has played “an absolutely critical role in the elucidation of membrane structure,” according to L. Andrew Staehelin (University of Colorado, Boulder, CO). Steere started with frozen samples and then combined a series of known methods—using an ultramicrotome for cutting, freeze drying to etch and expose surface features, and finally creating a replica with heavy metals. After Steere's initial demonstration of the...
The Rockefeller University Press
2005
The Rockefeller University Press
2005
You do not currently have access to this content.
