Correction: Elevating the level of Cdc34/Ubc3 ubiquitin-conjugating enzyme in mitosis inhibits association of CENP-E with kinetochores and blocks the metaphase alignment of chromosomes

Author and Article Information

Online ISSN: 1540-8140

Print ISSN: 0021-9525

The Rockefeller University Press

2002

J Cell Biol (2002) 158 (2): 371.

https://doi.org/10.1083/jcb.20020703154707

Topper et al. Vol. 154, No. 4, August 20, 2001. Pages 707–717.

In the original publication of this article, Table I contains a typographical error. In the first column under “Injection,” the third item should read “Cdc34 (≥3 mg/ml)” not “Cdc34CA (≥3 mg/ml).” The corrected version of the table is shown below.

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The Rockefeller University Press

2002

Table I.

Progression of microinfected cells through mitosis

Injection	Cell phase at injection	Number of cells injected	Number of cells arrested at prometaphase	Number of cells arrested at metaphase	Number of cells that completed mitosis
Buffer	Prophase	8			8
	Prometaphase	2			2
Cdc34CA (≥3 mg/mL)	Prophase	11			11
	Prometaphase	4			4
Cdc34 (≥3 mg/mL)	Prophase	104	89	5	10
	Prometaphase	24	18	5	1
	Late prometaphase	11		4	7^*
	Anaphase	6			6
Cdc34 (3 mg/mL) denatured	Prophase	6			6
	Prometaphase	3			3
Cdc34 (1.5 mg/mL)	Prophase	11	7		4
Cdc34 (3 mg/mL) with 10 μm MG132 or β-lactone	Prophase	15	15

Injection	Cell phase at injection	Number of cells injected	Number of cells arrested at prometaphase	Number of cells arrested at metaphase	Number of cells that completed mitosis
Buffer	Prophase	8			8
	Prometaphase	2			2
Cdc34CA (≥3 mg/mL)	Prophase	11			11
	Prometaphase	4			4
Cdc34 (≥3 mg/mL)	Prophase	104	89	5	10
	Prometaphase	24	18	5	1
	Late prometaphase	11		4	7^*
	Anaphase	6			6
Cdc34 (3 mg/mL) denatured	Prophase	6			6
	Prometaphase	3			3
Cdc34 (1.5 mg/mL)	Prophase	11	7		4
Cdc34 (3 mg/mL) with 10 μm MG132 or β-lactone	Prophase	15	15

These cells spent on average 32.5 min at metaphase, which is significantly different from uninjected cells (P < 0.05).

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Correction: Elevating the level of Cdc34/Ubc3 ubiquitin-conjugating enzyme in mitosis inhibits association of CENP-E with kinetochores and blocks the metaphase alignment of chromosomes

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Correction: Elevating the level of Cdc34/Ubc3 ubiquitin-conjugating enzyme in mitosis inhibits association of CENP-E with kinetochores and blocks the metaphase alignment of chromosomes

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