Nucleic acid-containing particles in the cytoplasm of Amoeba proteus (cf. reference 1) were counted after acridine orange staining. The number of particles per ameba was found to be correlated with cell age and size. Fresh daughters had a mean particle number of 5400, whereas predivision amebae contained around 11,000 particles. Amebae from two other strains contained similar particles. The particles were found to be clustered in fasted cells and redispersed after feeding. A marked increase in the particle population was noted in anucleate fragments. These results, together with those previously presented, suggest that the particles multiply intracellularly. Their nature and their relationship to previous work on nucleic acid labeling in Amoeba are discussed.
Article|
December 01 1962
CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS : II. On the Behavior and Possible Nature of the DNA-Containing Elements
M. Rabinovitch,
M. Rabinovitch
From the Department of Zoology, University of Wisconsin, Madison, Wisconsin.
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W. Plaut
W. Plaut
From the Department of Zoology, University of Wisconsin, Madison, Wisconsin.
Search for other works by this author on:
M. Rabinovitch
From the Department of Zoology, University of Wisconsin, Madison, Wisconsin.
W. Plaut
From the Department of Zoology, University of Wisconsin, Madison, Wisconsin.
Dr. Rabinovitch's permanent address is Faculty of Medicine, University of São Paulo, São Paulo, Brazil
Received:
July 10 1962
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright, 1962, by The Rockefeller Institute Press
1962
J Cell Biol (1962) 15 (3): 535–540.
Article history
Received:
July 10 1962
Citation
M. Rabinovitch, W. Plaut; CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS : II. On the Behavior and Possible Nature of the DNA-Containing Elements . J Cell Biol 1 December 1962; 15 (3): 535–540. doi: https://doi.org/10.1083/jcb.15.3.535
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