Coatomer is a cytosolic protein complex that forms the coat of COP I-coated transport vesicles. In our attempt to analyze the physical and functional interactions between its seven subunits (coat proteins, [COPs] alpha-zeta), we engaged in a program to clone and characterize the individual coatomer subunits. We have now cloned, sequenced, and overexpressed bovine alpha-COP, the 135-kD subunit of coatomer as well as delta-COP, the 57-kD subunit and have identified a yeast homolog of delta-COP by cDNA sequence comparison and by NH2-terminal peptide sequencing. delta-COP shows homologies to subunits of the clathrin adaptor complexes AP1 and AP2. We show that in Golgi-enriched membrane fractions, the protein is predominantly found in COP I-coated transport vesicles and in the budding regions of the Golgi membranes. A knock-out of the delta-COP gene in yeast is lethal. Immunoprecipitation, as well as analysis exploiting the two-hybrid system in a complete COP screen, showed physical interactions between alpha- and epsilon-COPs and between beta- and delta-COPs. Moreover, the two-hybrid system indicates interactions between gamma- and zeta-COPs as well as between alpha- and beta' COPs. We propose that these interactions reflect in vivo associations of those subunits and thus play a functional role in the assembly of coatomer and/or serve to maintain the molecular architecture of the complex.
Architecture of coatomer: molecular characterization of delta-COP and protein interactions within the complex.
- Views Icon Views
- PDF LinkPDF
- Share Icon Share
- Search Site
D Faulstich, S Auerbach, L Orci, M Ravazzola, S Wegchingel, F Lottspeich, G Stenbeck, C Harter, F T Wieland, H Tschochner; Architecture of coatomer: molecular characterization of delta-COP and protein interactions within the complex.. J Cell Biol 1 October 1996; 135 (1): 53–61. doi: https://doi.org/10.1083/jcb.135.1.53
Download citation file: