Hepatocyte Growth Factor (HGF, also known as Scatter Factor) is a powerful mitogen or motility factor in different cells, acting through the tyrosine kinase receptor encoded by the MET protooncogene. Endothelial cells express the MET gene and expose at the cell surface the mature protein (p190MET) made of a 50 kD (alpha) subunit disulfide linked to a 145-kD (beta) subunit. HGF binding to endothelial cells identifies two sites with different affinities. The higher affinity binding site (Kd = 0.35 nM) corresponds to the p190MET receptor. Sub-nanomolar concentrations of HGF, but not of a recombinant inactive precursor, stimulate the receptor kinase activity, cell proliferation and motility. HGF induces repairs of a wound in endothelial cell monolayer. HGF stimulates the scatter of endothelial cells grown on three-dimensional collagen gels, inducing an elongated phenotype. In the rabbit cornea, highly purified HGF promotes neovascularization at sub-nanomolar concentrations. HGF lacks activities related to hemostasis-thrombosis, inflammation and endothelial cells accessory functions. These data show that HGF is an in vivo potent angiogenic factor and in vitro induces endothelial cells to proliferate and migrate.
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1 November 1992
Article|
November 01 1992
Hepatocyte growth factor is a potent angiogenic factor which stimulates endothelial cell motility and growth.
F Bussolino,
F Bussolino
Department of Genetics, Biology and Medical Chemistry, University of Torino.
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M F Di Renzo,
M F Di Renzo
Department of Genetics, Biology and Medical Chemistry, University of Torino.
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M Ziche,
M Ziche
Department of Genetics, Biology and Medical Chemistry, University of Torino.
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E Bocchietto,
E Bocchietto
Department of Genetics, Biology and Medical Chemistry, University of Torino.
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M Olivero,
M Olivero
Department of Genetics, Biology and Medical Chemistry, University of Torino.
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L Naldini,
L Naldini
Department of Genetics, Biology and Medical Chemistry, University of Torino.
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G Gaudino,
G Gaudino
Department of Genetics, Biology and Medical Chemistry, University of Torino.
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L Tamagnone,
L Tamagnone
Department of Genetics, Biology and Medical Chemistry, University of Torino.
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A Coffer,
A Coffer
Department of Genetics, Biology and Medical Chemistry, University of Torino.
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P M Comoglio
P M Comoglio
Department of Genetics, Biology and Medical Chemistry, University of Torino.
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F Bussolino,
M F Di Renzo,
M Ziche,
E Bocchietto,
M Olivero,
L Naldini,
G Gaudino,
L Tamagnone,
A Coffer,
P M Comoglio
Department of Genetics, Biology and Medical Chemistry, University of Torino.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1992) 119 (3): 629–641.
Citation
F Bussolino, M F Di Renzo, M Ziche, E Bocchietto, M Olivero, L Naldini, G Gaudino, L Tamagnone, A Coffer, P M Comoglio; Hepatocyte growth factor is a potent angiogenic factor which stimulates endothelial cell motility and growth.. J Cell Biol 1 November 1992; 119 (3): 629–641. doi: https://doi.org/10.1083/jcb.119.3.629
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