The levels of type X collagen in mineralizing normal chicken epiphyses and nonmineralizing rachitic chicken tibial epiphyses were measured and compared. Qualitative immunoperoxidase studies with anti-chick type X collagen monoclonal antibodies on sections from normal and rachitic cartilage demonstrated that the type X collagen levels in rachitic growth plates are reduced. Northern hybridization of mRNA and biosynthetic studies have confirmed that type X collagen synthesis in rickets is also decreased. In hypocalcemic rickets, the level of type X collagen mRNA is reduced by 80% whereas the level of type X collagen mRNA is only reduced by 50% in normocalcemic rickets. These observations provide additional evidence that type X collagen is involved in the process of cartilage mineralization and also suggest that the partial recovery of type X collagen synthesis in normocalcemic rickets may be related to the elevated plasma concentration of calcium. Calcium concentration may therefore play an important role in the control of type X collagen synthesis.
Comparative studies of type X collagen expression in normal and rachitic chicken epiphyseal cartilage.
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A P Kwan, I R Dickson, A J Freemont, M E Grant; Comparative studies of type X collagen expression in normal and rachitic chicken epiphyseal cartilage.. J Cell Biol 1 October 1989; 109 (4): 1849–1856. doi: https://doi.org/10.1083/jcb.109.4.1849
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