A single enzyme found in both Drosophila and mammalian cells is able to selectively bind and degrade transforming growth factor (TGF)-alpha and insulin, but not EGF, at physiological concentrations. These growth factors are also able to inhibit binding and degradation of one another by the enzyme. Although there are significant immunological differences between the mammalian and Drosophila enzymes, the substrate specificity has been highly conserved. These results demonstrate the existence of a selective TGF-alpha-degrading enzyme in both Drosophila and mammalian cells. The evolutionary conservation of the ability to degrade both insulin and TGF-alpha suggests that this property is important for the physiological role of the enzyme and its potential for regulating growth factor levels.
Article|
September 01 1989
An evolutionarily conserved enzyme degrades transforming growth factor-alpha as well as insulin.
J V Garcia
,
J V Garcia
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
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B D Gehm
,
B D Gehm
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
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M R Rosner
M R Rosner
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Search for other works by this author on:
J V Garcia
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
B D Gehm
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
M R Rosner
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1989) 109 (3): 1301–1307.
Citation
J V Garcia, B D Gehm, M R Rosner; An evolutionarily conserved enzyme degrades transforming growth factor-alpha as well as insulin.. J Cell Biol 1 September 1989; 109 (3): 1301–1307. doi: https://doi.org/10.1083/jcb.109.3.1301
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