We report the sequence of a cDNA clone that codes for the carboxy-terminal portion of the peroxisomal protein, acyl-CoA oxidase, from the yeast, Candida tropicalis. This is a newly identified acyl-CoA oxidase sequence, most likely a second allele of POX4. The cDNA clone was expressed by in vitro transcription followed by translation. The major product, a 43-kD protein, associated with isolated peroxisomes in an in vitro import assay. More than half of the peroxisome-associated protein was protected from added protease, implying that it was internalized within the organelle. These findings indicate that there is sufficient information in the carboxy-terminal portion of the protein to target it to peroxisomes.
Article| July 01 1987
Import of the carboxy-terminal portion of acyl-CoA oxidase into peroxisomes of Candida tropicalis.
G M Small
P B Lazarow
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1987) 105 (1): 247–250.
G M Small, P B Lazarow; Import of the carboxy-terminal portion of acyl-CoA oxidase into peroxisomes of Candida tropicalis.. J Cell Biol 1 July 1987; 105 (1): 247–250. doi: https://doi.org/10.1083/jcb.105.1.247
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