Normal rat kidney cells infected with a Rous sarcoma virus (strain LA23) were used to study the dynamics of alpha-actinin-containing aggregates in transformed cells. Experiments were performed by microinjecting living cells with iodoacetamidotetramethylrhodamine alpha-actinin and allowing the fluorescent analogue to incorporate into cellular structures. Subsequent time-lapse recording indicated that the alpha-actinin-containing aggregates can undergo rapid formation, movement, and breakdown. In addition, experiments using the photobleaching recovery technique indicated that alpha-actinin molecules associated with the aggregates have a very high rate of exchange, whereas those associated with adhesion plaques in normal cells exchange much more slowly. The dynamic properties of alpha-actinin-containing aggregates may be closely related to the changes in cellular behavior upon oncogenic transformation.
Article| June 01 1987
Alpha-actinin-containing aggregates in transformed cells are highly dynamic structures.
S K Stickel
Y L Wang
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1987) 104 (6): 1521–1526.
S K Stickel, Y L Wang; Alpha-actinin-containing aggregates in transformed cells are highly dynamic structures.. J Cell Biol 1 June 1987; 104 (6): 1521–1526. doi: https://doi.org/10.1083/jcb.104.6.1521
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