Schwann cell proliferation induced by a myelin-enriched fraction was examined in vitro. Although nearly all the Schwann cells contained material that was recognized by antisera to myelin basic protein after 24 h, only 1% of the cells were synthesizing DNA. 72 h after the addition of the mitogen a maximum of 10% of the cells incorporated [3H]thymidine. If the cultures were treated with the myelin-enriched fraction for 24 h and then washed, the number of proliferating Schwann cells decreased by 75% when compared with those cells that were incubated with the mitogen continuously. When Schwann cells were labeled with [14C]thymidine followed by a pulse of [3H]thymidine 24 h later, every Schwann cell labeled with [3H]thymidine was also labeled with [14C]thymidine. Although almost every Schwann cell can metabolize the myelin membranes within 24 h of exposure, a small population of cell initially utilizes the myelin as a mitogen, and this population continues to divide only if myelin is present in the extracellular media. The percentage of the Schwann cells that initially recognize the myelin-enriched fraction as a mitogen is dependent upon the age of the animal from which the cells were prepared.
Article| March 01 1987
Developmental changes in myelin-induced proliferation of cultured Schwann cells.
J E Yoshino
P W Mason
G H DeVries
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1987) 104 (3): 655–660.
J E Yoshino, P W Mason, G H DeVries; Developmental changes in myelin-induced proliferation of cultured Schwann cells.. J Cell Biol 1 March 1987; 104 (3): 655–660. doi: https://doi.org/10.1083/jcb.104.3.655
Download citation file: