We have examined acetylcholine (ACh)-elicited potentials or currents in current- or voltage-clamped cultured myotubes exposed to 12-O-tetradecanoyl-phorbol-13-acetate (TPA), a potent tumor promoter that activates protein kinase C. Although this agent had little action on either membrane resting potential or electrical resistance, a reversible decrease in ACh sensitivity was induced on 3-4-d-old chick myotubes. Depression of transmitter action by TPA was extended to 7-8-d mouse myotubes only when they were treated with phosphatidylserine. Glyceryl dioleate had effects on myotubes similar to those of TPA but with a reduced efficacy. We conclude that the activation of protein kinase C might be involved with the capacity of ACh receptors to respond to transmitter stimulation.
Article| April 01 1985
Agents that activate protein kinase C reduce acetylcholine sensitivity in cultured myotubes.
B M Zani
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1985) 100 (4): 1339–1342.
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F Eusebi, M Molinaro, B M Zani; Agents that activate protein kinase C reduce acetylcholine sensitivity in cultured myotubes.. J Cell Biol 1 April 1985; 100 (4): 1339–1342. doi: https://doi.org/10.1083/jcb.100.4.1339
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