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Volume 10, Issue 4
1 August 1961
Content prior to 1962 was published under the journal name
The Journal of Biophysical and Biochemical Cytology
The Journal of Biophysical and Biochemical Cytology Cover Image for Volume 10, Issue 4
Article| August 01 1961

THE DIRECT ISOLATION OF THE MITOTIC APPARATUS

Daniel Mazia,
Daniel Mazia
From the Department of Zoology, University of California, Berkeley, California
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J. M. Mitchison,
J. M. Mitchison
From the Department of Zoology, University of California, Berkeley, California
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Heitor Medina,
Heitor Medina
From the Department of Zoology, University of California, Berkeley, California
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Patricia Harris
Patricia Harris
From the Department of Zoology, University of California, Berkeley, California
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Author and Article Information
Daniel Mazia
From the Department of Zoology, University of California, Berkeley, California
J. M. Mitchison
From the Department of Zoology, University of California, Berkeley, California
Heitor Medina
From the Department of Zoology, University of California, Berkeley, California
Patricia Harris
From the Department of Zoology, University of California, Berkeley, California
Received: March 23 1961
Copyright, 1961, by The Rockefeller Institute Press
1961
J Biophys and Biochem Cytol (1961) 10 (4): 467–474.
https://doi.org/10.1083/jcb.10.4.467
Article history
Received:
March 23 1961

A method for isolating the mitotic apparatus from dividing sea urchin eggs without the use of ethyl alcohol or of detergents is described. In the present method, the eggs are dispersed directly in a medium containing 1 M (to 1.15 M) sucrose, 0.15 M dithiodiglycol, and 0.001 M Versene at pH 6, releasing the visibly intact mitotic apparatus. The method is designed for studies of enzyme activities, lipid components, and the variables affecting the stability of the apparatus.

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Copyright, 1961, by The Rockefeller Institute Press
1961
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