To test the idea that cytochalasin retards actin assembly by binding to filament ends, we have designed a new assay for cytochalasin binding in which the number of filament ends can be varied independently of the total actin concentration. Actin is reacted with polylysine-coated polystyrene beads to make filament ends (Brown and Spudich, 1979, J. Cell Biol. 80:499-504) and then reacted with [3H]cytochalasin B. We have found that cytochalasin B binds to beads in the presence of actin, and that the number of cytochalasin B binding sites can be varied as a function of the number of filament ends independent of the total actin concentration by varying the bead concentration.
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Article| March 01 1981
Mechanism of action of cytochalasin: evidence that it binds to actin filament ends.
S S Brown
J A Spudich
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1981) 88 (3): 487–491.
S S Brown, J A Spudich; Mechanism of action of cytochalasin: evidence that it binds to actin filament ends.. J Cell Biol 1 March 1981; 88 (3): 487–491. doi: https://doi.org/10.1083/jcb.88.3.487
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