In continuous lines of cultured Drosophila cells two forms of cytoplasmic actin, designated II and III, are detected after a 25- to 120-min pulse label with [35S]methionine. However, only one of these, actin II, accumulates in the cell. With the use of a pulse-chase protocol and two-dimensional gel electrophoresis, it has been found that actin III is synthesized as a precursor of the more stable cytoplasmic actin II. The half-life of actin III was estimated to be approximately 50 min.
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Article| June 01 1979
A precursor of cytoplasmic actin in cultured Drosophila cells.
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1979) 81 (3): 680–683.
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E Berger, G Cox; A precursor of cytoplasmic actin in cultured Drosophila cells.. J Cell Biol 1 June 1979; 81 (3): 680–683. doi: https://doi.org/10.1083/jcb.81.3.680
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