Fibronectin isolated from cultures of chicken embryo fibroblasts (CEF) contains phosphorus linked to serine and threonine by monoester bonds. Normal and Rous sarcoma virus (RSV)-transformed cells were incubated with [32P]orthophosphate, and fibronectin was isolated from the cell surfaces and conditioned media. 32P was stably associated with fibronectin during immunoprecipitation, SDS-polyacrylamide gel electrophoresis, phospholipid solvent extraction, and hot acid but not alkaline treatment. After a limited acid hydrolysis of fibronectin, both phosphoserine and phosphothreonine were found. The specific radioactivity of the 32P-labeled fibronectin from the conditioned medium of normal CEF was higher than that from the cultures of transformed CEF.
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Article| March 01 1979
Fibronectin from chicken embryo fibroblasts contains covalently bound phosphate.
M H Teng
D B Rifkin
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1979) 80 (3): 784–791.
M H Teng, D B Rifkin; Fibronectin from chicken embryo fibroblasts contains covalently bound phosphate.. J Cell Biol 1 March 1979; 80 (3): 784–791. doi: https://doi.org/10.1083/jcb.80.3.784
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