Antibodies directed against whole histone and purified lysine-rich histone H1 extracted from isolated macronuclei of the ciliate Tetrahymena were obtained and conjugated to fluorescein isothiocyanate. The fluorescein-antibody conjugates were used to directly label Tetrahymena cells. Both macro- and micronuclei were visibly fluorescent in cells stained with anti-whole histone conjugate. However, the anti-H1 conjugate only labeled macronuclei. This in situ demonstration of the lack of positive immunofluorescent staining of micronuclei with anti-H1 conjugate provide further evidence for the absence of H1 in the genetically inactive, mitotically dividing Tetrahymena micronucleus.
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Article| October 01 1976
Immunofluorescence evidence for the absence of histone H1 in a mitotically dividing, genetically inactive nucleus.
C A Johmann
M A Gorovsky
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1976) 71 (1): 89–95.
C A Johmann, M A Gorovsky; Immunofluorescence evidence for the absence of histone H1 in a mitotically dividing, genetically inactive nucleus.. J Cell Biol 1 October 1976; 71 (1): 89–95. doi: https://doi.org/10.1083/jcb.71.1.89
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