A simple method is described for the freeze-fracture in situ of monolayer cultures grown on gold carriers coated with a thin layer of silicon monoxide. Preliminary observations on 3T3 mouse embryo fibroblasts indicate that this technique exposes large areas of cell membrane, making it possible to determine how areas of membrane specialization are related to the cell as a whole and to regions of cellular interaction. 3T3 cells cultured on silicon monoxide show no modification of growth properties compared to cells growing on Falcon plastic, and other cell lines also appear to grow well on this substrate.
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Article| December 01 1975
A simple method for freeze-fracture of monolayer cultures.
T R Collins
J C Bartholomew
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1975) 67 (3): 904–911.
T R Collins, J C Bartholomew, M Calvin; A simple method for freeze-fracture of monolayer cultures.. J Cell Biol 1 December 1975; 67 (3): 904–911. doi: https://doi.org/10.1083/jcb.67.3.904
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