Using a colloidal silica density gradient, HeLa cells in mitosis were found to have a density of 1.040–1.046 g/cc, lighter than the remaining interphase cells. The mitotic cells could be harvested and cultured after centrifugation, showing growth synchrony by measurement of a peak in mitotic index 21 hr after establishing the culture. By using Colcemid or vinblastine sulfate, HeLa cells were arrested in metaphase and centrifuged on the colloidal silica density gradient. The blocked metaphase cells were lighter in density than the interphase cells but somewhat more dense than untreated cells selected by the density gradient centrifugation. Near-equilibrium conditions were established during the centrifugation of cells so that cell density measurements could be made, and the gradient medium employed was not measurably toxic to those cells tested.
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December 01 1972
SEPARATION OF HELA CELLS BY COLLOIDAL SILICA DENSITY GRADIENT CENTRIFUGATION : I. Separation and Partial Synchrony of Mitotic Cells
David A. Wolff,
David A. Wolff
From the Department of Medical Chemistry and the Wallenberg Laboratory, University of Uppsala, Uppsala, Sweden.
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HÅkan Pertoft
HÅkan Pertoft
From the Department of Medical Chemistry and the Wallenberg Laboratory, University of Uppsala, Uppsala, Sweden.
Search for other works by this author on:
David A. Wolff
From the Department of Medical Chemistry and the Wallenberg Laboratory, University of Uppsala, Uppsala, Sweden.
HÅkan Pertoft
From the Department of Medical Chemistry and the Wallenberg Laboratory, University of Uppsala, Uppsala, Sweden.
Dr. Wolff's present address is the Department of Microbiology, The Ohio State University, Columbus, Ohio 43210.
Received:
June 19 1972
Revision Received:
August 04 1972
Online Issn: 1540-8140
Print Issn: 0021-9525
Copyright © 1972 by The Rockefeller University Press
1972
J Cell Biol (1972) 55 (3): 579–585.
Article history
Received:
June 19 1972
Revision Received:
August 04 1972
Citation
David A. Wolff, HÅkan Pertoft; SEPARATION OF HELA CELLS BY COLLOIDAL SILICA DENSITY GRADIENT CENTRIFUGATION : I. Separation and Partial Synchrony of Mitotic Cells . J Cell Biol 1 December 1972; 55 (3): 579–585. doi: https://doi.org/10.1083/jcb.55.3.579
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