Free cholesterol is demonstrated in formalin-fixed frozen sections when treated successively by digitonin, alcohol-ether, and the Schultz technique, in which circumstances cholesterol esters are not visualized. Cholesterol esters and free cholesterol are both demonstrated in comparable sections treated by the Schultz method alone, so that the difference between such sections indicates the sites at which cholesterol esters may be considered present.
Brief Notes| March 25 1956
A Method for the Histochemical Differentiation of Cholesterol and Its Esters
From the Departments of Pathology and Neurology, The Mount Sinai Hospital, and the College of Physicians and Surgeons, Columbia University, New York.
Received: November 22 1955
Copyright, 1956, by The Rockefeller Institute for Medical Research
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Irwin Feigin; A Method for the Histochemical Differentiation of Cholesterol and Its Esters . J Biophys and Biochem Cytol 25 March 1956; 2 (2): 213–214. doi: https://doi.org/10.1083/jcb.2.2.213
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