page 281, Di Cola and Robinson report in vivo evidence from protoplasts showing that a large fraction of the proteins that move part way through the channel end up back in the stroma.
The Tat system is required for transporting several protein components of a photosynthetic complex to the lumen of the chloroplast. For example, the 23K protein starts out in the cytoplasm as a 33-kD precursor, containing two NH2-terminal signal sequences. The first signal sequence is cleaved as the protein moves across the outer chloroplast membrane into the stroma, and the second as the protein passes through the Tat transport complex into the lumen.
When tobacco protoplasts were engineered to express a GFP-labeled pre-23K protein, both signal sequences were cleaved normally. However, only a small proportion of the mature protein ended up in the lumen, even when the researchers expressed minimal amounts of the construct.The peptidase for the second signal sequence faces the lumen. Di Cola and Robinson conclude that the fully processed 23K proteins detected in the stroma had to have reached the lumen, or at least proceeded part way through the Tat transport system, before they were sent back to the stroma.
When they blocked interaction between the GFP-labeled protein and the Tat system by mutating the recognition domain in the substrate protein, all of the GFP-labeled protein remained in the stroma as expected. But in this case the protein retained its second signal sequence, indicating that a stromal protease cannot cleave off the second signal sequence.
In vivo transport is likely to be more rather than less efficient than in vitro systems. The lack of the reexport ability in the in vitro system may reflect missing components—a theory that the researchers are now testing with add-back experiments. The reexport ability may represent a previously undetected quality control mechanism for thylakoid transport.